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Thus the action spectrum varies widely with reference to the variation of monochromatic light.
These amazing organisms are capable of capturing the energy of sunlight and fixing it in the form of potential chemical energy in organic compounds. The quantities are mind boggling. However, the photosynthetic efficiency decreases very dramatically with increasing wavelength beyond nm even though chlorophyll still absorbs light at these wavelengths. Finally, at high CO2 concentration, photosynthetic rate decreases due to: i Increased acidity of the mesophyll cells, ii A narcotic effect on the metabolic function of the cells, iii Closing of stomata, and iv Limitation of light for consumption of available CO2. Experiment 6. Fix the graduated tube with the zero mark exactly on the surface of the water and open the stopcock of the graduated tube to allow water to rise inside the tube up to the zero mark.
Measuring photosynthesis via the uptake of carbon dioxide Using 'immobilised algae' - It's easy and accurate to measure the rate of photosynthesis and respiration using immobilised algae in hydrogen carbonate indicator solution - known as the 'algal balls' technique. LED lamps produce less heat. You should see air coming out the sides of the disks. Glass-wares: Conical flasks, burette, pipette, measuring cylinder, beaker etc. The lab could be used in a unit on, the scientific method, a photosynthetic unit or in a unit on environmental problems global climate change and its affect on plants. There are difficulties with this method, as I'm sure you can appreciate.
Follow the information below to make "sinking plant disks". B Determination of Absorption Spectrum: 1.
The oxygen electrode is set up and calibrated in the usual way and, after calibration, move the pen to the centre of the chart paper with the zero control as O2 evolution is being measured. B Oxygen electrode assays for determination of Hill activity: The oxygen electrode is widely used in biochemical laboratories to monitor processes or reactions involving oxygen exchange. Repeat twice and calculate a mean bubble count — number of bubbles per minute. Plants are the primary producers. Make all connections air-tight thereafter.
The chloroplast suspension can be conveniently illuminated by a watt bulb.
Check the weed to see if it is bubbling.
Make sure enough disks are available to properly complete a controlled experiment. Hydrilla Sp 4.
Pick a testable question to answer when doing the experiment. The amplified current is fed to a chart recorder which gives a trace of the change in oxygen concentration with time.
There is a slight risk of infection from pond water, so take sensible hygiene precautions, cover cuts and wash hands thoroughly after the work is complete.